ABSTRACT
Background : Study of fibrilar, cellular and sub cellular structures of mineralized tissues is only possible after the removal of the calcium apatite of these tissues by the process of demineralization. Aims: The present study aims to evaluate six commonly used demineralizing agents to identify the best decalcifying agent. Materials and Methods: The present study included six different decalcifying solutions: 10% formal nitric acid, 8% formal nitric acid, 10% formic acid, 8% formic acid, Perenyi's fluid and Ethylene Di-Amine Tetra Acetic Acid. eight samples of posterior mandible of rat were decalcified in each of the decalcifying solutions and subjected to chemical end-point test. Ehrlich's Hematoxylin stain was used. Statistical Analysis Used: One way ANOVA was used for multiple group comparisons and Chi-square test was used for analyzing categorical data. P value of 0.05/less was set for statistical significance. Results: Samples treated with EDTA showed the best overall histological impression and the tissue integrity were well preserved. Formal nitric of both the percentages 10 and 8% gave fairly good cellular detail and were rapid in their action. Conclusion: The final impression led to the proposition that EDTA was indeed the best decalcifying agent available. However, with time constraint, the use of formal nitric acid is advocated.
ABSTRACT
Context: Lip prints are very useful in forensic investigation and personal identification. Like finger prints, even lip prints can be instrumental in identifying a person positively. Aims: Indians are closer to Mongoloids than to Caucasoids or Negroids as indicated by the phylogenetic tree. Most of the studies on lip prints are done in their own population. We have compared lip prints of Manipuris with other Indians (Aryans and Dravidians) who are both close to Mongoloid race and are genetically similar. Materials and Methods: A total of 100 students 50 males and 50 females were selected of whom 30 males and 30 females were of Aryan and Dravidian features and 20 males and 20 females showed the Mongol features. Study materials used were Red colored lipstick, Lip brush, Cellophane tape, White chart paper and Magnifying lens. The lip prints were analyzed by dividing them into eight compartments. Results: Analysis of lip prints showed that the most common and the least common pattern in both males and females (Aryans-Dravidians and Mongols) were the same, but the compartment wise distribution of the lip patterns was different. Conclusion: In the present study, it is established that there is no similarity of lip prints from one individual to another individual and between males and females. Regarding the comparison with Mongols, more studies with a larger sample size is necessary.